Akademik Veri Yönetim Sistemi
2. ULUSLARARASI MARDİN ARTUKLU BİLİMSEL ARAŞTIRMALAR KONGRESİ, Mardin, Türkiye, 23 - 25 Ağustos 2019, ss.114
Axillarin (5,7,3′,4′-tetrahydroxy-3,6-dimethoxy flavanol) is a methoxylated flavon-3-ol derivative found in Balsamorhiza macrophylla, Tanacetum vulgare, Pulicaria crispa, Filifolium sibiricum, Inula britannica, Wyethia bolanderi and the other Lamiaceae plants. Tanacetum alyssifolium is a local endemic species to Erzincan, Turkey. Axillarin was isolated previously from T. alyssifolium by our group and evaluated antiproliferative activity against HeLA cell lines (1). Here we report rapid and selective isolation process of axillarin. For this aim, the methanolic extract of plant material first fractioned over sephadex LH-20 using methanol as mobile phase. According to the TLC basis, axillarin containing fractions were combined and separated over C18 packed column. Water and methanol mixture were used as follows: 100:0, 80:20, 70:30 and 60:40 each 1 L. Axillarin was obtained from fractions eluted with 60:40 water: methanol system with high purity. In addition to, quantitative analysis of axillarin found in T. alyssifolium plant was done using HPLC-DAD. According to the results, the axillarin content of methanol extracts of plant material was found 17.88 mg/g extract. Using above isolation process, we achieved to isolation of 600 mg axillarin from 36 gr methanol extract. As a result, this process was suggested for large scale production of axillarin for further studies such as animal and human experiments.