Simultaneous Determination of Arbutin and Hydroquinone in Different Herbal Slimming Products by Gas Chromatography-Mass Spectrometry

dursunoğlu b., yuca h., GÖZCÜ S., YILMAZ B., güvenalp z.

International Multidisciplinary Symposium on Drug Research and Development, Erzurum, Turkey, 5 - 07 October 2017, pp.97

  • Publication Type: Conference Paper / Full Text
  • City: Erzurum
  • Country: Turkey
  • Page Numbers: pp.97
  • Erzincan Binali Yildirim University Affiliated: No


Arbutin (4-hydroxyphenyl-β-D-glucopyranoside) is a phenolic glucoside that is found mainly in Ericaceae and Saxifragaceae species [1]. Hydroquinone is 1,4-dihydroxybenzene and the metabolite of arbutin [2]. In this study, a simple, precise, sensitive and specific method has been developed for the simultaneous determination of arbutin and hydroquinone in different herbal slimming products containing Calluna vulgaris by gas chromatography-mass spectrometry (GC-MS). The retention times of arbutin and hydroquinone were found 11.32 and 5.44 min, respectively. The linear range in this developed method was 5-500 ng/mL for arbutin and hydroquinone. The intra- and inter-day precisions, expressed as the relative standard deviation, were less than 1.94 and 2.73%, determined from quality control samples for arbutin and hydroquinone, and accuracy was within 1.13 and 2.56% in terms of relative error, respectively. Limit of detection and quantification for arbutin were 0.555 and 1.665 ng/mL, for hydroquinone 0.031 and 0.093 ng/mL, respectively. The developed method can be used for routine quality control analysis of arbutin and hydroquinone in different herbal slimming products.