Determination of the Effect of Tebuconazole Applications on Cucumber (Cucumis sativus L.) Seedling with Morphological and Molecular Methods

Bulut H., Öztürk H. İ., Dursun A.

Kahramanmaraş Sütçü İmam Üniversitesi Tarım ve Doğa Dergisi, vol.24, no.5, pp.969-977, 2021 (ESCI)


Aim of this study was to determine the effect of tebuconazole applications on cucumber seedling via morphological and molecular methods. In order to determine the most appropriate dose of different concentrations (25, 50, 75, 100, 125 and 150 ppm) of tebuconazole affecting the seedling quality and preventing possible genotoxic effects, retrotransposon motility at all doses was determined. Polymorphism rates and changes in GTS values were also determined. At the applied doses, polymorphism values were found as 0%, 5.55%, 16.66%, 38.88%, 55.55% and 61.11%, respectively. The GTS value was decreased from 100% to approximately 38.89% based on the applied doses. Statistical evaluations indicated that different dose applications resulted significant changes in the seedling characteristics. The seedling and stem heights were suppressed by 57.5% and 55.9%, respectively compared to the controls. The stem diameter increased 9.95% following the application. Overall, 50, 75 and 150 ppm doses were determined to achieve an increase in the dry matter and leaf chlorophyll content. In this study, applications of Tebuconazole at different doses controled the seedling height in cucumber and affected specific quality characteristics of the seedlings.