Akademik Veri Yönetim Sistemi
2. International Congress on Analytical and Bioanalytical Chemistry-ICABC 2020, Antalya, Türkiye, 11 - 14 Mart 2020, ss.106-107
In this study, it was aimed to create a metabolic profile of the drug from plasma samples of rats given orally solifenacin for 10 days. After the extracts obtained from control and drug applied rat plasma samples were dissolved in the mobile phase, they were analyzed in positive ion mode by LC/QTOF-MS. For the chromatographic separation of metabolites, a gradient elution program with a mobile phase consisting of a mixture of acetonitrile containing 0.1% formic acid and ultrapure water containing 0.1% formic acid was used. Data analysis, metabolic pathway analysis and chemometric analysis results of LC/QTOF-MS data of plasma samples were reported. The 43 metabolites that show significant differences in plasma levels of 4441 features determined by mass detector are identified by HMDB and METLIN. It was demonstrated with OPLS-DA that the metabolic profiles of the drug and control groups differ from each other with good selectivity and sensitivity.