Molecular Characterization of Some Squash (Cucurbita pepo L.) Genotype Growing from Erzincan province of Turkey by Simple Sequence Repeat (SSR) Markers


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Öztürk H. İ. , Bulut H., Dönderalp V., Korkut R.

ULUSLARARASI MÜHENDİSLİK VE DOĞA BİLİMLERİ ÇALIŞMALARI KONGRESİ(ICENSS), Ankara, Turkey, 7 - 09 May 2021, vol.1, pp.163

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.163

Abstract

Plant genetic resources constitute the most valuable resources of countries. It is of great importance to determine the genetic variation between these resources and to use them in breeding studies accordingly. 29 squash genotypes collected in order to determine the genetic diversity of some snack gourd genotypes widely cultivated in Erzincan were examined. SSR (Simple Sequence Repeat) markers were used to determine the genetic diversity of these genotypes. Polymorphism rate was determined to be 100% in all primers in the evaluation made using SSR markers. The 7 SSR markers used produced a total of 15 polymorphic bands, the number of alleles per marker ranged from 2 (GMT-P41, GMT-M61, GMT-M259, GMT-P18, GMT-P25 and GMT-M30 markers) to 3 (GMTP68 markers). changed and the average number of alleles was found to be 2.14. Gene diversity per marker varied between 0.064 and 0.314 for GMT-M61 and GMT-P41 markers. The expected heterozygosity of the SSR locus ranged from 0.068 (CMTp18, CMTm61) to 0.422 (CMTp41), with a mean of 0.192. The mean of Nei gene diversity was (h) 0.477. Polymorphic information content (PIC) varied between 0.06 (GMT-M61) and 0.247 (GMT-P41), and the average PIC value per marker was found to be 0.152. In the clustering analysis using Nei's genetic distance, it was determined that 29 genotypes were divided into 3 main groups. Genetic relationships calculated based on molecular genetic data can be used in planning squash breeding programs for future studies by determining the degree of diversity in the population.