Investigation of the Effects of Pomiferin Substance Isolated from Maclura pomifera Plant on Indomethacin-Induced Gastric Damage in Rats


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Thesis Type: Postgraduate

Institution Of The Thesis: Ataturk University, Eczacılık Fakültesi, Temel Eczacılık Bilimleri, Turkey

Approval Date: 2015

Thesis Language: Turkish

Student: İlyas BOZKURT

Principal Supervisor (For Co-Supervisor Theses): Bünyami Mesut Halıcı

Co-Supervisor: Esra Dilek

Abstract:

Aim: Previously, water extract of Maclura pomifera plant has been found effective on gastric damage. In this study, Pomiferin substance with possible curative activity was purified and its antiulcer effects were investigated on gastric damage induced by indomethacin in rats. Material and Method: Pomiferin substance needed was purified from Maclura pomifera plant by TLC method and its structure was elucidated by NMR methods. For the study, a total of 36 Sprague Dawley male rats were purchased and they were divided into 6 groups (n=6 each). The 25 mg/kg indomethacin was administered orally to all groups, except healthy ones, and 25 mg/kg ranitidine (Ulcuran® 25 mg/ml vial) to REF group, 100 mg/kg pomiferin to PMF-100 group, 200 mg/kg pomiferin to PMF-200 group and 300 mg/kg pomiferin to PMF-300 group. Rats were sacrificed after 6th hours of administrations and ulcerative fields were identified in stomach via graph papers. Biochemically, LPO and GSH levels as well as SOD and CAT activities were measured in stomach tissues. Results: Comparing with healthy group, in control group, it was observed that while ulcerative area, LPO levels and CAT activity increased significantly (p<0.05), SOD and GSH levels decreased simultaneously (p<0.05). It was observed that in REF group and PMF groups dose-dependently dropped the increased areas of ulcer, LPO levels and CAT activity (p<0.05), It was found that GSH levels as well as SOD activities significantly regenerated (p<0.05). Conclusion: It was found that pomiferin purified from Maclura pomifera plant was effective on gastric injury induced by indomethacin. This effect was thought to be caused by positive changes in the antioxidant system.