Teucrium chamaedrys L. ve Achillea biebersteinii Afan. bitkilerinin antioksidan aktivitelerinin mukayesesi


Thesis Type: Postgraduate

Institution Of The Thesis: Erzincan Binali Yildirim University, Fen-Edebiyat Fakültesi, Kimya Bölümü, Turkey

Approval Date: 2012

Thesis Language: Turkish

Student: HÜSEYİN KANBUR

Supervisor: Ekrem Köksal

Open Archive Collection: AVESIS Open Access Collection

Abstract:

This study is aimed to examine the antioxidant activities of Teucrium chamaedrys L. and Achillea biebersteinii Afan. plants. For this purpose, plants were collected from Çilhorozu which is the village of Çayırlı, a town of Erzincan province, and they were dried and ethanol extractions were prepared. On prepared ethanol extraction, total antioxidant activity was determined by ferroccyanate reduction method; Cu2+ and potassium ferric thiocyanate were determined by Cuprac method; was determined by reduction method capacity of Fe3+ ions; the activitiy of scavenging of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) was determined; the total amount of the compounds of phenolic and flavonoid was determined. It was observed that 30 ? g mL-1 concentrated of ethanol extracts obtained from Teucrium chamaedrys L. and Achillea biebersteinii Afan. plants inhibited the lipid peroxidation of linoleic acid emulsion as 77,2 % and 71,3 % respectively. In the same concentration, ?-tocoferol and trolox caused inhibitions of 85,3 % and 83,1 %, respectively. Teucrium chamaedrys L. plant in which it was detected that the content of total phenolic were much more than those in Achillea biebersteinii Afan. Plant still displayed higher DPPH scavenging and metal reduction activity. Butylated hydroxy toluene (BHT), ethylenediamine tetraacetic acid(EDTA), ?-tocopherol and an analoge of its which is the solved in water, namely Trolox were used as reference antioxidant compounds, while the antioxidant activity of Teucrium chamaedrys L. and Achillea biebersteinii Afan. plants whose ethanol extractions were taken out were being examined.This study is aimed to examine the antioxidant activities of Teucrium chamaedrys L. and Achillea biebersteinii Afan. plants. For this purpose, plants were collected from Çilhorozu which is the village of Çayırlı, a town of Erzincan province, and they were dried and ethanol extractions were prepared. On prepared ethanol extraction, total antioxidant activity was determined by ferroccyanate reduction method; Cu2+ and potassium ferric thiocyanate were determined by Cuprac method; was determined by reduction method capacity of Fe3+ ions; the activitiy of scavenging of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) was determined; the total amount of the compounds of phenolic and flavonoid was determined. It was observed that 30 ? g mL-1 concentrated of ethanol extracts obtained from Teucrium chamaedrys L. and Achillea biebersteinii Afan. plants inhibited the lipid peroxidation of linoleic acid emulsion as 77,2 % and 71,3 % respectively. In the same concentration, ?-tocoferol and trolox caused inhibitions of 85,3 % and 83,1 %, respectively. Teucrium chamaedrys L. plant in which it was detected that the content of total phenolic were much more than those in Achillea biebersteinii Afan. Plant still displayed higher DPPH scavenging and metal reduction activity. Butylated hydroxy toluene (BHT), ethylenediamine tetraacetic acid(EDTA), ?-tocopherol and an analoge of its which is the solved in water, namely Trolox were used as reference antioxidant compounds, while the antioxidant activity of Teucrium chamaedrys L. and Achillea biebersteinii Afan. plants whose ethanol extractions were taken out were being examined.