INVESTIGATION THE IN VITRO EFFECTS OF SOME ANTIBIOTICS GIVEN TO PATIENTS AFTER SURGERY ON PARAOXONASE-I ENZYME ACTIVITY PURIFIED FROM HUMAN SERUM


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Thesis Type: Postgraduate

Institution Of The Thesis: Erzincan Binali Yildirim University, Fen Bilimleri Enstitüsü, Turkey

Approval Date: 2018

Thesis Language: Turkish

Student: Aycan YILMAZ

Supervisor: Esra Dilek

Abstract:

Paraoxonase (PON1; E.C.3.1.8.1) is an enzyme from the group of calcium-dependent A-esterases associated with high-density lipoprotein (HDL), also called aryldialylphosphatase synthesized in the liver. In this study, PON1 enzyme was purified from human serum with ammonium sulfate precipitation (in the range of 60-80%), DEAE-Sephadex A-50 ion exchange chromatography and Sephadex G-200 gel filtration chromatography steps to be approximately 322,6 fold with 47,5% yield. The purity of the enzyme was controlled by SDS polyacrylamide gel electrophoresis to determine the molecular mass of about 43 kDa. In our study, the effects of some antibiotic drugs (fosfomycin, cefixime, cefuroxime, cefaclor monohydrate) given after surgery on human serum PON1 enzyme activity were investigated in vitro. IC50 values and Ki constants of these drugs were determined. It was observed that inhibition of enzyme activity was effected by low inhibition mechanisms. It was observed that cefixim from these antibiotics decreased more in vitro PON1 activity.