Alıç (Crataegus monogyna) bitkisinin yapraklarının antioksidan aktivitesinin belirlenmesi


Thesis Type: Postgraduate

Institution Of The Thesis: Erzincan Binali Yildirim University, Fen-Edebiyat Fakültesi, Kimya Bölümü, Turkey

Approval Date: 2012

Thesis Language: Turkish

Student: EMRAH DİKİCİ

Supervisor: Ekrem Köksal

Open Archive Collection: AVESIS Open Access Collection

Abstract:

This study had been aimed to examine the antioxidant activitie of leaves of hawthorn (Crataegus monogyna). For this purpose, leaves of hawthorn (crataegus monogyna) was collected from Bağıştaş village which İliç, a town of Erzincan province in May 2011. It was dried and ethanol extraction was prepared. On prepared ethanol extraction, total antioxidant activity was determined by ferroccyanate reduction method, reduction capacity of Cu2+ was determined by Cuprac method, reduction capacity of Fe3+ ions were determined by thiocyanate reduction method, the activitiy of scavenging of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) was determined and was determined the total amount of the compound of phenolic and flavonoid. It was observed that 10 ? g mL-1 concentrated of ethanol extract obtained from hawthorn (Crataegus monogyna) plant inhibited the lipid peroxidation of linoleic acid emulsion as 40.9 % respectively. In the same concentration, Butylated hydroxy toluene (BHT) and trolox caused inhibitions of 68.3% and 29.9 %, respectively. The content of total phenolic activity, DPPH scavenging and metal reduction activity of hawthorn (Crataegus monogyna) which was detected more than trolox. Butylated hydroxy toluene (BHT), ?-tocopherol and its an analoge which is solved in water, namely Trolox, were used as reference antioxidant compounds, while the antioxidant activity of hawthorn (Crataegus monogyna) plant whose ethanol extraction was taken out was examined.