İliç papatyası (Tanacetum Alyssifolium) bitkisinden izole edilen sekonder metabolitlerin antioksidan ve antikanser aktivitelerinin incelenmesi

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Thesis Type: Postgraduate

Institution Of The Thesis: Erzincan Binali Yildirim University, Fen-Edebiyat Fakültesi, Kimya Bölümü, Turkey

Approval Date: 2017

Thesis Language: Turkish

Student: YAKUP ULUTAŞ

Supervisor: Ekrem Köksal

Open Archive Collection: AVESIS Open Access Collection

Abstract:

This study was carried out with the aim of isolation of secondary metabolites present in Tanacetum alyssifolium (Bornm.) Grierson plant belonging to Tanacetum genus, which is represented by 161 species in the world and 47 species in Turkey, by means of chromatographic methods and determination of structures by spectroscopic methods as well as determination of their antioxidant and antiproliferative activities. For this purpose, Tanacetum alyssifolium plants was collected from the foothills of Munzur Mountains in Erzincan province and dried at room temperature. The upper part of ground plant was extracted with ethyl acetate/ n-butanol and water solvent system. The n-butanol extract was subjected to the procedure of column chromatography. The structures of the isolated compounds were elucidated by spectroscopic methods (1 H-NMR, 2 D-NMR, HPLC-TOF) to reveal that Luteolin-7-O-β-D-glikozit and fraxidine. The antioxidant activities of the isolated compounds were evaluated according to the DPPH and ABTS free radical scavenging and metal ion chelating capacities. Antiproliferative activities of the samples was investigated on HeLa (Human Rectum Cancer) and C6 (Rat Brain Tumor) cell lines at four different concentrations (5, 25, 50, 100 μg/ml) and compared with 5-Fluorouracil (5-FU), which is used as an anticancer drug.